Department of Microbiology

Overview

Microbiology is a discipline that involves the study of bacteria, viruses, parasites, fungi and immunology. The greatest achievements in medical sciences has attributed to the development in diagnostics, treatment and prevention of several Infectious diseases. Microbiology with fundamentals on infectious diseases contributed immensely to the prevention and management of many communicable diseases.

Infection Control Committee plays a vital role in preventing hospital acquired infections (HAI) and setting guidelines for sterilization, Biomedical Waste Management, antimicrobial stewardship, vaccination programs etc., the scope of Clinical Microbiology continues to expand with control and prevention of emerging and re-emerging infections. The Department has a strong commitment in Research and Undergraduate – Postgraduate education.

It is housed in modern facilities with interactive faculty with strengths in pathogenic microbiology, environmental microbiology and microbial genetics. The Department also has excellent core facilities with social impact of services rendered to the patients. One of the major achievements of our department is the establishment of Molecular Diagnostics and Research Laboratory (MDRL) in the year 2020.

In our Microbiology Central Laboratory, we do perform a wide array of tests to detect bacteria, fungi, parasites, viruses with varied advanced serological and automated technologies. We have extended our scope of services rendered to the patients greatly and give round the clock services to provide better quality care for our patients.

Our Microbiology Central Laboratory is managed by a dedicated, efficient team of medical microbiologist and well trained, proficient lab technicians for timed bench works. A sound computer based hospital information system is used to manage the patients’ sample right from receiving in the lab till reporting. All the patient laboratory reports are issued adhering to the turn-around-time. Critical alerts are reported to the concerned departments immediately.

Our lab is equipped with state of the art diagnostic facilities such as automated blood culture system (BACT/ ALERT), automated identification and antimicrobial susceptibility testing system (Vitek-2), PCR, Fluorescent microscope along with other conventional microbiological tools for diagnostic services.

Academics

  • M.B.B.S (Bachelor of Medicine and Bachelor of Surgery)

    Clinical applications are discussed and correlated while teaching each session in Microbiology for undergraduates. Innovative methods such as concept mapping, seminar, group discussions, self study and hand outs are used for small group teaching. Demonstration and hands on training with individual attention provided for practical exposure. As an additional curriculum enrichment, value added courses and elective courses are conducted periodically.

  • M.D.(Doctor of Medicine)

    The Post Graduate course in M.D. Microbiology was started in 2004 with intake of four PG’s per batch and till now thirteen of them have graduated and working in various reputed medical colleges, diagnostic laboratories and research laboratories across the country. Training is given to make them a good clinical Microbiologist, which makes them an expert from lab to bed side.

    Strenuous training is given in various fields like bacteriology, mycology, virology, parasitology , hospital infection control and infectious diseases.

  • M.Sc.(Master of science)

    M.Sc Medical Microbiology, a three year post graduate program was commenced in the Department of Microbiology to provide a comprehensive theoretical knowledge in medical and molecular aspects of immunology, mycology, parasitology, virology and bacteriology to infection which includes the spread, disease causation, diagnosis and/ or treatment of pathogens of major significance to public health, and advanced practical training in this diverse field. Since 2008, thirteen batches of students were graduated for this course.

  • Student's practical laboratory

    Spacious student’s laboratory with 140 Binocular Microscope for routine practical activities.

    Student’s practical laboratory has state of the art facilities which facilitates the students to learn and perform experiments.

  • Museum

    Departmental museum provides the knowledge and experience to pursue a better career.  Around 100 handmade models, charts, illustrations and original specimens of parasites are available in our centre.

    Museum exhibits several models, charts, illustrations and specimens, which enhances the learning and understanding of the subject.

  • Research

    The department offers excellent opportunities for research in various arenas of microbial diseases. Sixteen PhD students are enrolled with the Department of Microbiology including six scholars who have completed their research work. Department has published n number of research articles in various national and international high-indexed journals.

Central Diagnostic Laboratory

  • Chemiluminescene Immuno Assay (CLIA):

    Diagnostic technology is rapidly evolving, and over the last decade, substantial progress has been made even for the identification of antibodies, increasingly approaching this type of diagnostic to that of automated clinical chemistry laboratory. The wide dynamic range, greater than that of immunoenzymatic methods, the high sensitivity and specificity of the results expressed in quantitative form, the high degree of automation and the clinical implications related to the reduction in the turnaround time, and the ability to run a large number of antibody tests (even of different isotypes), directed towards large antigenic panels in random access mode. It also brings together critical performance factors of accuracy, efficiency and uptime with patented enabling technologies to empower the lab.

  • Enzyme Linked Immunosorbant Assay:

    The basic enzyme-linked immunosorbent assay (ELISA), or enzyme immunoassay (EIA), is distinguished from other antibody-based assays because separation of specific and non-specific interactions occurs via serial binding to a solid surface, usually a polystyrene multiwell plate, and because quantitative results can be achieved.

    The ELISA procedure results in a colored end product which correlates to the amount of analyte present in the original sample.

    ELISAs are quick and simple to carry out, and since they are designed to rapidly handle a large number of samples in parallel, they are a very popular choice for the evaluation of various research and diagnostic targets.

    ELISAs were first developed in the early 1970s as a replacement for radioimmunoassays.

    They remain in wide use in their original format and in expanded formats with modifications that allow for multiple analytes per well, highly sensitive readouts, and direct cell-based output.

  • Nephelometry:

    Nephelometry is defined as the detection of light energy scattered or reflected toward a detector that is not in the direct path of the transmitted light. A nephelometer or aerosol photometer is an instrument for measuring the concentration of suspended particulates in a liquid or gas colloid. A nephelometer measures suspended particulates by employing a light beam (source beam) and a light detector set to one side (often 90°) of the source beam.

  • Biosafety cabinet Class II:

    Class II Bio safety Cabinet Type A1 & A2 are designed for protection of operator, product and environment from pathogenic agents and microorganisms transmitted in respiratory way and also for creation of a bacterial dust free air environment. It has a common plenum from which 30% of air is exhausted, and 70% re-circulated to the work area as the down flow. Type A cabinets exhaust air directly back to the laboratory, and they may contain positive pressure contaminated plenums.

  • Vitek 2 Compact system:

    Vitek 2 Compact system:

    Today’s global healthcare challenges like Multi-Drug Resistant Organisms (MDRO) mean that microbiology labs need to be flexible and responsive to provide the right information at the right time. The efficiency of the VITEK® 2 COMPACT instrument and VITEK® 2 PC software offers you the capacity to help improve therapeutic success and patient outcomes1 through reliable microbial identification (ID) and antibiotic susceptibility testing (AST). The instrument also lets you enhance laboratory efficiencies with reduced hands-on time and rapid reporting capabilities. All this, in a cost-effective, space-saving design.

  • BacT/alert

    BacT/alert

    BACT/ALERT 3D is used for detecting the presence or absence of microorganisms in Blood & Sterile body fluids

    BACT/ALERT 3D saves time, facilitates cross-training and helps prevent errors. This system offers immediate bottle recognition, in control of bottle loading and unloading and virtually eliminating bottle handling errors during microbial detection testing. The system's automatic, built-in quality control, along with a low false positive rate and a rapid response time of this system, means we can do more in less time with greater accuracy.

    The latest generation of BACT/ALERT Culture Media brings the most advanced, innovative microbial growth and detection technology to our laboratory. BACT/ALERT media provide unsurpassed performance, in the detection of a wide variety of microorganisms including bacteria, fungi, and yeasts.

Molecular Diagnostics and Research Laboratory

  • Real Time PCR: RT-PCR

    Real Time PCR: RT-PCR can be used for epidemiological, diagnostic and research identifications of nucleic acids. Few examples are as follows) Reliable genotyping of virus isolates is essential to understand the evolution, virulence and transmission of SARS-CoV-2 and emerging variants. Moreover, during the respiratory virus season, when there is a significant overlap between symptoms, it is critical to accurately discriminate between the co-circulating viruses by multiplex, real-time PCR. Especially in the recent trends, SARS-CoV-2, flu A/B and RSV A/B epidemiology can be done.

    ii) Gene expression analysis

    • Analyzing gene expression by quantifying the corresponding mRNA or lncRNA is critical to many areas such as cancer and disease research and biomarker identification. For quantification of known targets, reverse transcription qPCR (RT-qPCR) remains the gold standard method, providing fast analysis from just a few to many genes

    iii) miRNA quantification and profiling

    • MicroRNAs (miRNAs) have emerged as potential biomarkers for numerous diseases and biological processes, such as cancer, heart disease, nervous system function, hemostasis and DNA repair.

    iv) DNA quantitation

    • Quantitation of DNA targets plays an important role in many research areas: from detection of genetically modified organisms (GMO) in food to the analysis of genetic variations such as single nucleotide polymorphisms (SNPs), copy number variations (CNV) and somatic mutations.

  • -20 degree and -80 degree Deep Freezer:

    -20 degree and -80 degree Deep Freezer:

    Ultra-Low temperature are devices intended for the safe storage of vaccines, human cells, tissues and other laboratory samples at ultra-low temperatures ranging from -20°C to -86°C. Designed for the safe storage of our specimens, the Ultra-Low Freezers are equipped with a high-performing cooling system that ensures temperature uniformity, reliability and energy efficiency. All our Ultra-Low Freezers are equipped with a 24*7 real time monitoring solution.

  • NABL Accredited

    NABL Accredited for:

    • SARS-CoV-2

    • HCV Qualitative PCR

Hospital Infection Control

  • Hospital Infection Control Committee

    • Infection control committee was initiated from  2016

    • Performing well established with the implementation of ICC protocols.

    • Handled COVID 19 pandemic so good and  received an award by APIC for best COVID  MANAGEMENT all over INDIA

  • Specials initiative Activities of HICC  during covid pandemic

    • NABL accredited Molecular Diagnostics and Research  Laboratory establishment for COVID 19 diagnosis.

    • COVID fever clinic establishment.

    • Donning and Doffing classes every week during covid  pandemic  for the HCWs posted in COVID wards.

    • Active screening and isolation of covid positives and  contact tracing of suspected covid HCWs with the help  of community medicine department.

    • Introduction and usage of Ibhar app  for hand hygiene  and Biomedical waste audit and other audits

Team Microbiology

  • DR. P. Neelusree MBBS, M.D. (Professor and Head)

    • Active member in Indian Association of Medical Microbiologists

    • Chairman of Hospital Infection Control Committee, SMCH

    • Completed Curriculum Implementation Support Programme & Revised Basic Course in Medical Education

    • Committee Member in Board of Studies for Post Graduate and Undergraduate

    • Course Coordinator for overall M.Sc. Post graduates

    • Published 13 research articles in reputed National and International Journals

    • Area of Interest: Infection Control

  • Dr. S. Jayakumar MBBS, M.D. (Professor)

    • Completed Basic Course in Medical Education

    • Published 17 National and three International research articles in high indexed Journals.

    • Area of Interest: Bacteriology

  • Dr. L. Premkumar MBBS, M.D (Associate Professor)

    • Best Faculty II Phase MBBS Award (Thrice) students choice in ACE awards (2019,2020,2021)

    • Member Secretary in Hospital Infection Control Committee, SMCH

    • Completed Revised Basic Course in Medical Education

    • Published four National and two International research articles in reputed Journals.

    • Area of Interest: Hospital Infection Control

  • Dr. E. Subbalakshmi MBBS, M.D (Associate Professor)

    • Active member in Indian Journal of Medical Microbiology (IJMM) & Indian Academy of Tropical Parasitology (IATP)

    • Authored “Preparatory Manual for Physiotherapy students” and “Practical Manual of Clinical Microbiology for Post Graduates”

    • Ethical Committee Member in Sri Lalithambigai Medical College and Hospital

    • Underwent dedicated training in the field of Molecular Microbiology from KIPM, Chennai.

    • Published 11 research articles in reputed National and International Journals.

    • Area of Interest: Antimicrobial Resistance, Infection Control

  • Dr. Tasneembanu MBBS, M.D (Associate Professor)

    • Completed Curriculum Implementation Support Programme & Revised Basic Course in Medical Education

    • Published 8 research articles in reputed National and International Journals.

    • Area of Interest: Antimicrobial stewardship

  • Dr. D. Aruna MBBS, M.D (Assistant Professor)

    • Completed Curriculum Implementation Support Programme in Medical Education

    • Published 11 research articles in reputed National and International Journals.

    • Area of Interest: Antimicrobial Resistance, Infection Control

  • Dr. Abirama Sundari MBBS, M.D (Assistant Professor)

    • Hospital Infection Control Committee Officer, SMCH

    • Completed Revised Basic Course in Medical Education

    • Trained in Simulation based “Basics of Infection Prevention & Control.

  • Dr. G. Bhuvaneshwari M.Sc, Ph. D Medical Microbiology (Assistant Professor)

    • Committee Member in Ph.D. Admission and Methodology Examination

    • Reviewer in International Journal- “Plant Cell Biotechnology and Molecular Biology”, “International Journal of Tropical Disease and Health” and “ Journal of Pharmaceutical Research International”

    • Authored “MCQ in Microbiology”

    • Bagged prizes in numerous oral and poster presentations in various Conferences.

    • Published 26 research articles in reputed National and International Journals.

    • Area of Interest: Molecular Diagnostics

  • Dr. Harish MBBS, M.D, FIID (Tutor)

    • Receiver of Young Scientist Award from Malaysian University

    • Fellow in Infectious diseases from the University of South Wales (UNSW), Sydney.

    • Invited speaker at American Conference on Medicine and Health Sciences, New York.

    • Presented several papers at National and International conferences.

    • Underwent special training in Hospital Infection Control from JIPMER.

    • Underwent dedicated training in the field of Molecular Microbiology from SRMC, Chennai.

    • Area of Interest: General infectious diseases, Antimicrobial resistance and Hospital infection control

  • Mrs. Pooja Nair M.Sc., Medical Microbiology (Tutor)

    • Co-authored chapter in Manual of Lab Investigations in Rheumatic Diseases, JayPee Publishers.

    • Completed NPTEL-AICTE Faculty Development Course in Applied Environmental Microbiology

    • Published six research articles in national and International Journals.

    • Area of interest: Immunology and Molecular diagnostics